Wolves face La Liga opposition in the form of Espanyol, while Old Firm rivals Celtic and Rangers have been paired with FC Copenhagen and Braga
Arsenal must overcome Olympiacos to remain in contention for Europa League glory, while Manchester United will face Club Brugge in the last 32.
Wolves’ continental adventure has delivered another intriguing tie, with Nuno Espirito Santo’s side set to take on Espanyol.
Celtic, meanwhile, will face Danish opposition in the form of FC Copenhagen and their Old Firm rivals Rangers tackle Braga.
Elsewhere, Ajax – Champions League semi-finalists in 2018-19 – will face another La Liga side in the form of Getafe while Bayern Leverkusen take on Porto.
Record winners Sevilla, with five successes to their name, take on Cluj and Inter will lock horns with Ludogorets.
Arsenal made their way to the final of the competition last season, before coming unstuck against London neighbours Chelsea.
The Gunners will be hoping to go one better 12 months on, but face a tough test in the first knockout round against Greek giants Olympiacos.
Manchester United will feel as though the draw has been a little kinder to them, but Club Brugge have already faced Real Madrid and Paris Saint-Germain in the Champions League this term.
Ole Gunnar Solskjaer has spoken of his desire to secure major silverware for the Red Devils and could follow in the footsteps of predecessor Jose Mourinho by savouring Europa League glory.
There are, however, a number of other heavyweight outfits still in the mix.
Wolves have held their own since returning to continental competition and will fancy their chances of troubling Espanyol.
Celtic and Rangers are locked in another battle for domestic supremacy in Scotland, but will divert their attention when taking on FC Copenhagen and Braga respectively.
Antonio Conte’s Inter, given the quality at their disposal, will be among the favourites to go all the way and should have too much for Ludogorets.
Ajax, given their rich European history, will also be heavily fancied in their meeting with Getafe.
Sevilla have shown in the past that they have the formula when it comes to the UEFA Cup/Europa League and Cluj are unlikely to be relishing a clash with the Liga outfit.
Shakhtar Donetsk against Benfica pitches two giants from their respective nations against one another, while another Portuguese side, in the form of Sporting, will face Istanbul Basaksehir.
Bundesliga rivals Eintracht Frankfurt and Wolfsburg have been paired with Red Bull Salzburg and Porto respectively, while Bayer Leverkusen face a tough test against Porto.
The final fixtures at this stage see Roma take on Gent, APOEL tackle FC Basel and AZ Alkmaar face Austrian side LASK.
The first legs will take place on February 20, 2020 and the return dates will be held a week later.
Europa League last-32 draw in full:
Wolves vs Espanyol
Sporting vs Istanbul Basaksehir
Getafe vs Ajax
Bayer Leverkusen vs Porto
FC Copenhagen vs Celtic
APOEL vs FC Basel
Cluj vs Sevilla
Olympiacos vs Arsenal
AZ Alkmaar vs LASK
Club Brugge vs Manchester United
Ludogorets vs Inter
Eintracht Frankfurt vs Red Bull Salzburg
Shakhtar Donetsk vs Benfica
Wolfsburg vs Malmo
Roma vs Gent
Rangers vs Braga
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